Investigator’s Annual Reports (IAR’s) for Crater Lake National Park
Hybridization in an isolated population: an example from Penstemon (Scrophulariaceae)
Report Number: 12835
Reporting Year: 1998
Permit Number: CRLA199802-002
Date Received: Jan 01, 1999
Principal Investigator: Shannon Datwyler, Department of Evolution, Ecology and Organismic Biology, Ohio State University, Columbus, OH
Park-assigned Study Id. #: CRLA199802-002
Permit Expiration Date: Jan 01, 2000
Permit Start Date: Jan 01, 1998
Study Starting Date: Jan 01, 1997
Study Ending Date: Jan 01, 1999
Study Status: Completed
Activity Type: Research
Subject/Discipline: Plant Communities (Vegetation)
Objectives: To investigate the patterns and frequency of natural hybridization between Penstemon rupicola and Penstemon davidsonii var. davidsonii on Wizard Island, Crater Lake National Park, with special focus on the potential for hybrid speciation in an isolated habitat.
Findings and Status: Currently, I am investigating the gene flow patterns observed in three different hybrid swarms on Wizard Island based on both morphological and molecular data. Preliminary results indicate a unidirectional pattern of gene flow from P. rupicola into P. davidsonii based on sampling from the 1997 field collections. In the 1998 field season I increased sampling of hybrid swarms to add two new populations (total of three populations for both 1997 and 1998 field seasons).
Leaf tissue was collected from a total of 68 hybrid plants representing two hybrid swarms on the island in addition to 12 individuals of P. rupicola and 24 individuals of P. davidsonii. In addition to sampling on the island, five populations (12 individuals per population, 60 total individuals) were sampled at various points around the western portion of the rim of the lake in order to examine population structure of these populations and to subsample the genetic variation existing around the rim. For each population sampled, voucher specimens were collected that will be deposited at Ohio State University. ;Pollinator observations were also made and several insect pollinators were collected for identification.
Two Bombus species were collected after being observed visiting Penstemon flowers and three leafcutter bees (Megachilidae) were also collected. In addition to these pollinators, hummingbirds and syrphid flies were observed to visit Penstemon flowers but were not collected. ;In addition to investigations of gene flow patterns in hybrid swarms, I have initiated a study to develop molecular markers that can be used to study gene flow distances within populations using microsatellite markers in paternity analysis of seeds. Seed capsules were collected from 60 individual plants representing two hybrid swarms (20 capsules from one population, 40 from the other). Currently, these seeds are being germinated and will be used for DNA extractions following germination. The pollen donor for each seed will then be determined by paternity analysis to calculate gene flow distances within and between populations. Preliminary pollinator observations suggest that bumblebees may facilitate pollen flow over long distances because of long flights between flower visits, whereas hummingbirds were observed to travel very short distances between floral visits, such as visiting the nearest neighbor plant.
These observations suggest that pollen flow would be limited in populations that are visited largely by hummingbirds and may be greater in populations visited largely by bumblebees. These predictions can be tested with the paternity study. ;The final year of investigation (1999) will be focused on augmenting the pollinator observations collected in 1998 as well as elucidating the underlying factors related to the pattern of unidirectional gene flow that I have observed, including experiments testing pollen tube growth rates in interspecific crosses.
For this study, were one or more specimens collected and removed from the park but not destroyed during analyses? Yes
Funding provided this reporting year by NPS: 0
Funding provided this reporting year by other sources: 1500
Full name of college or university: n/a
Annual funding provided by NPS to university or college this reporting year: 0
Other pages in this section
- Index – Principal Investigators
- Index – Research Projects By Year
- 12685 – Botrychium pumicola Annual Inventory
- 12686 – Dutton Ridge Air Visibility Monitoring
- 12687 – Spotted Owl Inventory, Crater Lake National Park
- 12688 – Departure Analysis
- 12689 – Magma Chamber Evolution of Cascade Volcanoes
- 12690 – Plant materials for Crater Lake National Park Revegetation Project
- 12691 – Volcanic evolution of the Crater Lake Region
- 12692 – Tree Section Collection
- 12693 – Fire Monitoring Plots
- 12694 – RAWS Installation
- 12695 – Origin of the Magma Chamber Under Mount Mazama
- 12696 – Trail impact monitoring
- 12697 – North American Truffling Society Foray
- 12698 – Establishment and Defense of the Cascade Range Forest Reserve
- 12699 – Fine Particulate Monitoring
- 12700 – Volcanic Breccias: Evaluation of Fragment and Deposit Origins
- 12701 – Developing a Resource Database for Crater Lake
- 12702 – U.S. Geological Survey-Hydrologic Benchmark Station
- 12703 – Visibility Monitoring
- 12704 – Treespade Transplanting Trails
- 12705 – A survey of the aquatic macroinvertebrates
- 12706 – Ecology of kokanee salmon and rainbow trout
- 12707 – Limnological Studies of Crater Lake
- 12708 – Particle fluxes in Crater lake and their relationship to nutrient cycling
- 12709 – Studies of hydrothermal processes in Crater Lake, Oregon
- 12710 – Effects of Climate on Physical and Chemical Characteristics of Crater Lake
- 12711- Collection of Representative Rocks from Crater Lake
- 12712 – Research Spotted Owl Distribution
- 12713 – What Price Expansion? Dams Versus the National Park Concept
- 12714 – Administrative History of John Day Fossil Beds National Monument
- 12715 – Plant Materials for Crater Lake National Park Revegetation Project
- 12716 – Volcanic Breccias: Evaluation
- 12717 – Reserved Water Rights Claim Preparation for the Klamath Basin Adjudication
- 12718 – Fine Particulate Monitoring. NPS Contract Number CX0001-8-0017
- 12719 – A Proposed Gas-Volcanic Solution To the Crater Lake, Oregon Collapse Structure
- 12720 – Volcanic Evolution of the Crater Lake Region
- 12721 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12722 – Ecology of Kokanee Salmon and Rainbow Trout
- 12723 – Fungi Inventory of Crater Lake National Park
- 12724 – Hydrologic Studies
- 12725 – Limnological Studies of Crater Lake
- 12726 – Crater Lake National Park 10-year Limnological Studies Final Report
- 12727 – A Population Survey for the Rare Sedge Carex Whitneyi at Crater Lake National Park
- 12728 – National Benchmark Station
- 12729 – PSD Increment Tracking
- 12730 – Prescribed Fire
- 12731 – Fire Monitoring Plots
- 12732 – Hazardous Fuel Pile and Burn
- 12733 – Preparation for Klamath River Basin Adjudication
- 12734 – Volcanic Evolution of the Crater Lake Region
- 12735 – Fine Particulate Monitoring
- 12736 – Plant Materials for Crater Lake National Park Revegetation Project
- 12737 – Ecology of Kokanee Salmon and Rainbow Trout in Crater Lake, Oregon
- 12738 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12739 – Amphibian Survey in Crater Lake National Park
- 12740 – Longterm Limnological Monitoring
- 12741 – A population survey for the rare sedge Carex whitneyi at Crater Lake National Park
- 12742 – National Benchmark Station, Collection of Basic Records
- 12743 – PSD Increment tracking
- 12744 – Prescribed Fire
- 12745 – Fire Monitoring Plots
- 12746 – Hazardous Fuel Pile and Burn
- 12747 – Preparation for Klamath River Basin Adjudication
- 12748 – Volcanic Evolution of the Crater Lake Region
- 12749 – Fine Particulate Monitoring. NPS Contract Number CX0001-8-0017
- 12750 – Plant Materials for Crater Lake National Park Revegetation Project
- 12751 – Ecology of Kokanee Salmon and Rainbow Trout in Crater Lake, Oregon
- 12752 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12753 – Amphibian Survey in Crater Lake National Park
- 12754 – Longterm Limnological Monitoring
- 12755 – Conservation Biology of the Mt. Mazama Collomia
- 12756 – Checklist of Plants of Crater Lake National Park
- 12757 – Spotted Owl Inventory
- 12758 – Crater Lake National Park Vegetation Mapping
- 12759 – Fire Monitoring Plots
- 12760 – Hazardous Fuel Pile and Burn
- 12761 – Prescribed Fire
- 12762 – Volcanic Evolution of the Crater Lake Region
- 12763 – Spotted Owl Inventory
- 12764 – Conservation Biology of the Mt. Mazama Collomia
- 12765 – Amphibian Survey in Crater Lake National Park
- 12766 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12767 – Ecology of Kokanee Salmon and Rainbow Trout in Crater Lake, Oregon
- 12768 – Fine Particulate Monitoring. NPS Contract Number CX0001-8-0017
- 12769 – Crater Lake National Park Vegetation Mapping
- 12770 – PSD Increment tracking
- 12771 – Plant Materials for Crater Lake National Park Revegetation Project
- 12772 – Longterm Limnological Monitoring
- 12773 – Botrychium pumicola Annual Inventory
- 12774 – National Benchmark Station, Collection of Basic Records
- 12775 – A population survey for the rare sedge Carex whitneyi at Crater Lake National Park
- 12776 – Checklist of Plants of Crater Lake National Park
- 12777 – Fire Monitoring Plots
- 12778 – Hazardous Fuel Pile and Burn
- 12779 – Management Ignited Fire
- 12780 – Volcanic Evolution of the Crater Lake Region
- 12781 – Restoration Strategies for Collomia mazama-Phase II
- 12782 – Conservation Biology of the Mt. Mazama Collomia
- 12783 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12784 – Ecology of Kokanee Salmon and Rainbow Trout in Crater Lake, Oregon
- 12785 – Fine Particulate Monitoring. NPS Contract Number CX0001-8-0017
- 12786 – Crater Lake National Park Vegetation Mapping
- 12787 – Survey of the hydra (coelenterata) in Crater Lake
- 12788 – Climate Change and Lower Food Web Structure in Lake Ecosystems
- 12789 – PSD Increment tracking
- 12790 – Federal Highway Admin HQ-Rim Revegetation Project
- 12791- Revegetation Lodge Landscape
- 12792 – Ecology of Pumice Desert
- 12793 – Longterm Limnological Monitoring
- 12794 – Crater Lake Tributaries Flow Assessment
- 12795 – Northern Spotted Owl Inventory
- 12796 – Botrychium pumicola Monitoring Project at Llao Rock (5th year)
- 12797 – Preparation for Klamath River Basin Adjudication
- 12798 – National Benchmark Station, Collection of Basic Records
- 12799 – Hydrothermal Convection in Cascade Volcanoes-Magma Migration in Cascade Volcanoes
- 12800 – Phylogenetics and Speciation in a Daphnia pulex Species Complex
- 12801 – Restoration strategies for Collomia mazama – phase II
- 12802 – The use of camera bait stations to detect fisher and wolverine in Crater Lake National Park
- 12803 – Fire Monitoring Plots
- 12804 – Ecology of Kokanee Salmon and Rainbow Trout in Crater Lake, Oregon
- 12785 – Fine Particulate Monitoring. NPS Contract Number CX0001-8-0017
- 12786 – Crater Lake National Park Vegetation Mapping
- 12787 – Survey of the hydra (coelenterata) in Crater Lake
- 12788 – Climate Change and Lower Food Web Structure in Lake Ecosystems
- 12789 – PSD Increment tracking
- 12790 – Federal Highway Admin HQ-Rim Revegetation Project
- 12791 – Revegetation Lodge Landscape
- 12792 – Ecology of Pumice Desert
- 12793 – Longterm Limnological Monitoring
- 12794 – Crater Lake Tributaries Flow Assessment
- 12795 – Northern Spotted Owl Inventory
- 12796 – Botrychium pumicola Monitoring Project at Llao Rock (5th year)
- 12797 – Preparation for Klamath River Basin Adjudication
- 12798 – National Benchmark Station, Collection of Basic Records
- 12799 – Hydrothermal Convection in Cascade Volcanoes-Magma Migration in Cascade Volcanoes
- 12800 – Phylogenetics and Speciation in a Daphnia pulex Species Complex
- 12801 – Restoration strategies for Collomia mazama – phase II
- 12802 – The use of camera bait stations to detect fisher and wolverine in Crater Lake National Park
- 12803 – Fire Monitoring Plots
- 12804 – Hazardous Fuel Pile and Burn
- 12805 – Management Ignited Fire
- 12806 – Volcanic Evolution of the Crater Lake Region
- 12807 – Restoration Strategies for Collomia mazama-Phase II
- 12808 – Ecology of Asarum species
- 12809 – Physical Characteristics of the Cleetwood Air-fall Tephra
- 12810 – Conservation Biology of the Mt. Mazama Collomia
- 12811 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12812 – Ecology of Kokanee Salmon and Rainbow Trout in Crater Lake, Oregon
- 12813 – Fine Particulate Monitoring. NPS Contract Number CX0001-8-0017
- 12814 – Crater Lake National Park Vegetation Mapping
- 12815 – Survey of the hydra (coelenterata) in Crater Lake
- 12816 – Climate Change and Lower Food Web Structure in Lake Ecosystems
- 12817 – Federal Highway Admin HQ-Rim Revegetation Project
- 12818 – Revegetation Lodge Landscape
- 12819 – Potential for hybridization and gene flow in an isolated population of Penstemon (Scrophulariaceae)
- 12820 – Nutrient study of Crater Lake
- 12821 – Ecology of Pumice Desert
- 12822 – Survey of Freshwater Ostracoda (Crustacea) in Crater Lake National Park
- 12823 – 1997 EMAP Oregon Pilot Survey
- 12824 – Longterm Limnological Monitoring
- 12825 – Crater Lake Tributaries Flow Assessment
- 12826 – Archeological Excavation of Bear Creek Cave
- 12827 – Survey Fort Klamath-Union Creek military wagon road within boundaries of Park
- 12828 – Botrychium pumicola Monitoring Project at Llao Rock (5th year)
- 12829 – Preparation for Klamath River Basin Adjudication
- 12830 – National Benchmark Station, Collection of Basic Records
- 12831 – Northern Spotted Owl Inventory
- 12832 – Scenic Images of Crater Lake National Park: A Focus Group Analysis
- 12833 – Hydrothermal Convection in Cascade Volcanoes-Magma Migration in Cascade Volcanoes
- 12834 – Phylogenetics and Speciation in a Daphnia pulex Species Complex
- 12836 – FIA/FHM Inventory of eastern Oregon’s timberlands
- 12837 – Educational Research for Elementary School Science
- 12838 – Trace element partitioning coefficients for feldspar in high-silica rhyolite
- 12839 – Volcanic Evolution of the Crater Lake Region
- 12840 – Restoration Strategies for Collomia mazama-Phase II
- 12841 – Inventory of Native Bumblebees of Crater Lake
- 12842 – Source constraints for growth of continental arcs based on Re-Os isotope fractionation
- 12843 – Bull Trout Restoration in Crater Lake National Park, Oregon
- 12844 – Inventory of Native Bumblebees of Crater Lake
- 12845 – Magma migration and Hydrothermal Convection in High Cascade volcanoes
- 12846 – Volcanic Evolution of the Crater Lake Region
- 12847 – Stable Isotopic Study of Sulfur Degassing during the Eruption of Mt. Mazama
- 12848 – Examination of the factors promoting hybridization between Penstemon davidsonii and P. rupicola
- 12849 – Soil Survey of Crater Lake National Park
- 12850 – Multi-spectral Thermal Imager (MTI)
- 20572 – Calibration of the (U-Th)/He geochronometer by analysis of the Mt. Mazama ash flow
- 20805 – USEPA’s Environmental Monitoring and Assessment Program (EMAP)
- 21365 – Conduit processes of the Cleetwood eruption, inferred from textural, petrologic, and water content analyses
- 21726 – Forestry inventory and Analysis
- 21728 – Seasonal effects of prescribed fire at Crater lake
- 22081 – Transport of Asiatic Aerosols into Crater Lake National Park
- 22333 – Assessment of Sensitive Amphibians in Southwest Oregon (State – Federal Partnership)
- 23891 – The Generation of Post-Caldera Rhyodacite in Crater Lake, Oregon
- 24578 – Ecology of the Pumice Desert
- 24750 – Inventory of Amphibians and Reptiles Klamath Parks Network Inventory and Monitoring Program
- 24831 – Forestry inventory and Analysis
- 24892 – Territorial Interactions Between Species of Corvids
- 25031 – Intercontinental Transport and Chemical Transformation Experiment (ITCT)
- 25854 – Fire History of Whitebark Pine Forests Crater Lake, Mount Rainier, and North Cascades National Parks
- 26142 – Effects of Prescribed Burning on Mycorrhizal Fungi in Crater Lake National Park
- 27032 – Intercontinental Transport and Chemical Transformation Experiment (ITCT)
- 27732 – Small Mammal Inventories in Klamath Network
- 28174 – The role of western dwarf mistletoe in fire susceptibility and behavior in mature ponderosa pine stands
- 28176 – The role of western dwarf mistletoe in fire susceptibility and behavior in mature ponderosa pine stands
- 28845 – Forestry inventory and Analysis
- 28949 – Seasonal Effects of Prescribed Fire on Ponderosa Pine
- 29581 – Soil Characterization for 2006 International Soil Science Society
- 29629 – REVSYS: A Holistic Approach to a Holarctic Group
- 29969 – Effects of Prescribed Burning on Mycorrhizal Fungi in Crater Lake National Park
- 30792 – Distribution and abundance of bat species in Crater Lake and others
- 31048 – Crater Lake NP Winter Wolverine Survey 2004 – 2005
- 32052 – Bryophyte Survey and Inventory in the Crater Lake Caldera
- 32134 – The role of western dwarf mistletoe in fire susceptibility and behavior in mature ponderosa pine stands
- 32344 – Patterns and consequences of insect herbivory in Lupinus lepidus at Crater Lake National Park
- 32739 – Intercontinental Transport and Chemical Transformation Experiment (ITCT)
- 33788 – Spatial, Temporal and Depth Distribution of Snow Properties in the Crater Lake Snowpack
- 34486 – Forest Inventory and Analysis
- 35198 – Crater Lake NP Winter Wolverine Survey 2004 – 2005
- 35203 – Fire History of Whitebark Pine Forests Crater Lake, Mount Rainier, and North Cascades National Parks
- 35760 – Distribution and relative abundance of bat species in Crater Lake National Park and others
- 35832 – Western Airborne Contaminants Assessment Project
- 35873 – 2005 Crater Lake NP Rangeland Ecological Site Description Data Collection
- 35944 – The Evolution of Reproductive Isolation in Mimulus
- 36142 – Evolution of color patterns in garter snakes
